Related article: *Weiss, F. a. American T3'pe Culture Collection, 2029 M Street, N.W., Washington, D.C. *Members of Committee on Bacteriological Technic, 1955-7 tDeceased Preface This manual is intended for use in those types of microbiological work which involve the study of microbial cultures or of viruses, either for identification or for learning the properties of the organisms investigated. This book takes the place of Biaxin Xl Pak the loose-leaf publication issued during the period of 1923-1956 under the name of ''Manual of Methods for Pure Culture Study of Bacteria." The present manual covers a wider scope but still includes the subject of ''pure culture study, " the meaning of which is discussed. Biaxin Xl Pac The methods given here are not to be regarded as official. The com- mittee has always taken the stand that official methods should not be adopted in the case of research work, because it is continually necessary to modify research methods in order to keep them up to date. The standardization of methods tends to hinder the development of new technics, while the chief function of this committee is to stimulate its development. The methods in this manual, therefore, are merely claimed to be those regarded as satisfactory by the committee at the time of publication. Whenever practical, the Biaxin Xl Filmtab methods have been tested by the committee in comparison with other procedures. Of the chapters in this book, V, VIII, IX, X, and XI are almost entirely new. The others are revisions, more or less complete, of leaflets of the old manual. In the case of these revised Buy Biaxin Xl chapters, the names of the contributors at the chapter heads are the latest revisers, not the original authors. In the case of the five entirely new chapters, however, the actual authors are cited at the chapter heads. H. J. Conn M. J. Pelczar, Jr. Vll Contents Preface Biaxin Xl 500 Mg Vll Chapter I. Introductory. Precautions. Practical Hints Chapter II. Staining Methods. General Principles. General Bacterial Stains. Negative Staining of Bacteria. The Gram Stain. Acid-fast Staining. Spore Staining. Staining the Diphtheria Organism. Fla- gella Staining. Capsule Stains. Stain for Fat Droplets. Robinow's Stains for Nuclear Apparatus. Stains for Spirochaetes. Dye Solubil- ities, by H. J. Conn, J. W. Bartholomew, and M. W. Jennison 10 Chapter III. Preparation of Media. Introductory. Cultivation and Storage Media. Enrichment Media. Differential Media. Media for Determination Biaxin Xl Antibiotic of Physiological Properties. Media for Special Bacteriological Procedures. Media for Special Purposes. Appendix: Specifications for Bacteriological Grade Agar, Gelatin, and Peptone. byG. W. Burnett, M. J. Pelczar, Jr. and H.J. Conn 37 Chapter IV. The Measurement of pH, Titratable Acidity, and Oxidation- Reduction Potentials. The Measurement of pH. Titratable Acidity. Buffer Action, and pH Adjustment of Culture Media. The Measure- ment of Oxidation-Reduction Potentials, by Barnett Cohen ... 72 Chapter V. Maintenance and Preservation of Cultures. Introduc- tion. Selection of Materials. Dormant Purchase Biaxin Online Conservation. Maintenance Methods. Media Commercially Available. Media Recommended. Biaxin Filmtab 500mg by Freeman A. Weiss 99 Chapter VI. The Study of Obligately Anaerobic Bacteria. Anaerobic Culture Methods and Equipment. Technics for the Study of Anaer- obic Bacteria, by L. S. McClung and Robert B. Lindberg .... 120 Chapter VII. Routine Tests for the Identification of Bacteria. Intro- duction. The Descriptive Charts. Determining Optimum Condi- tions for Growth. Incubation. Variation. Micromethods. Cul- X CONTENTS tural Characteristics. Study of Cell Morphology. Relation to Free Oxygen. Action on Nitrates. Chromogenesis. Indole Production. The Production of Hydrogen Sulfide. Liquefaction of Gelatin. Cleavage of Sugars, Alcohols, and Glucosides. Hydrolysis of Starch. The Methyl Red and Voges-Proskauer Tests. Acid Production in Milk. Rennet Production, by H. J. Conn, Buy Cheap Biaxin M. W. Jennison, and 0. B. Weeks 140 Chapter VIII. Physiological and Biochemical Technics. Biaxin Xl Pack Introduction. Growth Measurements. Preparations of Cells and Extracts. Bio- chemical Technics, by R. D. DeMoss and R. Order Biaxin Online C. Bard Biaxin Bid 500mg 169 Chapter IX. Serological Methods. Introduction. Preparation of Anti- serum. Agglutination. Precipitation. Complement-fixation Test